Tesco is ending its 24-hour opening hours at 76 stores across the UK as the company looks to be more "responsive to the way customers want to shop".
Affected stores will shut between midnight and 6am, with the change being rolled out between 14 March and 11 April 2016.
The supermarket, which is part-way through a turnaround plan under chief executive Dave Lewis, said it would allow staff to be "freed up to replenish products on the shelves overnight, which will mean better availability of products and better looking stores when they open their doors to customers each morning".
"Being responsive to the way customers want to shop in store means making a much more efficient use of Tesco time and resources, making savings for Tesco which can be passed on to customers," the grocer explained,
Tony Hoggett, retail director for Tesco, added: "We’re always thinking about how to serve our customers better in each of our stores and with the growth of online grocery shopping, these stores saw very few customers during the night.
“It makes much more sense for colleagues at those stores to focus on replenishing the shelves instead and making sure they’re fully stocked when they open their doors at 6am.
“We’ll continue to make changes in store in ways that will make shopping at Tesco a better experience for our customers, at the times they want to shop.”