The 15-year contract will commence on 1 April next year and covers a section of road called Area 13, which is located in Cumbria and North Lancashire.
Kier will provide routine and cyclic maintenance, defect repairs, emergency incident response and severe weather services.
The firm is also on track to secure a two-year contract to provide maintenance services for Areas 6 and 8 of Highways England's network, covering East Anglia and the East of England. This shorter contract would start at the same time as the Area 13 agreement.
Kier Group's chief executive, Haydn Mursell, said:
We are pleased to have secured the Area 13 contract and look forward to the completion of the negotiations around Areas 6 and 8.
These awards reflect Kier's ability to tailor its services to Highways England's evolving delivery model, as well as maintain the group's leadership in the growing UK highways management and maintenance market.
The group's share price was up 1.2 per cent in early morning trading to 1,410.3p.
Kier Group posted a pre-tax loss of £15m in the year ended 30 June due to one-off costs, such as those relating to its acquisition of rival Mouchel, down from a profit of £39m last year.