The 250,000 sq ft shop, which anchors the city’s new Grand Central retail development, has created 650 new jobs across Birmingham.
Andy Street, managing director, John Lewis said: “Today sends a clear message that John Lewis is firmly committed to the British High Street and that physical shops remain as important to us as they do to our customers.
“It is particularly exciting that customers will be able to step directly from New Street Station, into four floors of inspiring fashion, home and technology.”
Lisa Williams, head of the Birmingham branch, added: “Birmingham is a city that has always led the way, so it feels right that Birmingham is home to our most innovative shop to date.
“My team and I are looking forward to welcoming customers to what is a unique retail space.”