Wasps fly-half Danny Cipriani has been selected for England's tour of South Africa in June after being called upon by Eddie Jones for the first time.
Elsewhere there are eight uncapped players in the 34-man squad, including New Zealand-born flanker Brad Shields, Cipriani's Wasps teammate Dan Robson and Sale Sharks centre Cameron Redpath.
Cipriani, who won the last of his 14 England caps in 2015, was included after altering his game on Jones' advice in the Premiership.
Jones insisted Cipriani, who last started a match for England a decade ago and has until now been ignored by the Australian, hadn't been recalled.
"He hasn't had a recall, I've never picked him," said Jones.
"I've picked him for the first time. I've watched him play closely, I think he deserves the opportunity. He's done some things I've asked him to do in games. I'm happy to give him the opportunity."
Cipriani will have to dislodge Owen Farrell and George Ford before sealing a starting spot for England, with the former having been named captain in the place of the injured Dylan Hartley.
"He's got to prove he's the best 10 in the squad," said Jones. "Or the best 15. We wouldn't have picked him if we didn't think [he could do] so."
The inclusion of Shields, who developed through the New Zealand Rugby (NZR) system and plays for Super Rugby franchise the Hurricanes, has caused controversy.
Shields has been made an exception to England's policy of not selecting players playing abroad, while NZR have said they are "extremely disappointed" in the decision.
"[Shields is a] good, hard working player," said Jones.
"He plays for the best team in the southern hemisphere. He's a considerable influence in their team, a team that has a lot of skill and talent. He's a hard working, gritty player.
"He's a great back-up for Chris Robshaw for us."
Backs: Mike Brown (Harlequins), Danny Cipriani (Wasps), Elliot Daly (Wasps), Nathan Earle (Saracens), Owen Farrell (Saracens), George Ford (Leicester Tigers), Alex Lozowski (Saracens), Jonny May (Leicester Tigers), Cameron Redpath (Sale Sharks), Dan Robson (Wasps), Henry Slade (Exeter Chiefs), Denny Solomona (Sale Sharks), Ben Spencer (Saracens), Ben Te'o (Worcester Warriors), Ben Youngs (Leicester Tigers).
Forwards: Luke Cowan-Dickie (Exeter Chiefs), Tom Curry (Sale Sharks), Ben Earl (Saracens), Ellis Genge (Leicester Tigers), Jamie George (Saracens), Jonny Hill (Exeter Chiefs), Nick Isiekwe (Saracens), Maro Itoje (Saracens), Joe Launchbury (Wasps), Joe Marler (Harlequins), Chris Robshaw (Harlequins), Brad Shields (Hurricanes/Wasps), Sam Simmonds (Exeter Chiefs), Kyle Sinckler (Harlequins), Billy Vunipola (Saracens), Mako Vunipola (Saracens), Harry Williams (Exeter Chiefs), Jack Willis (Wasps), Mark Wilson (Newcastle).