The company behind the Shard's viewing platform has launched a lawsuit against three so-called urban climbers who scaled the building in July.
In a statement today The View from the Shard, which is on floors 68-72 of the building, said the three individuals had climbed from its open air viewing gallery on level 72 into the "spire" of the building.
According to the company, in recent months commercial buildings, hospitals, utility suppliers and sporting venues have experienced a "surge" in such incidents.
"The activities of these individuals cause significant disruption to businesses, the emergency services and the general public," it said.
"They also pose a serious risk to themselves."
Patrick Allen, the company's chief executive, added: "The act of free-running or 'Parkour' is strictly prohibited. We will not tolerate unnecessary risk to the safety of our staff, visitors and the general public."
In a separate incident, in July 2013 six Greenpeace activists protesters climbed from the roof of London Bridge station to the summit of the Shard and unveiled a banner reading "Save the Arctic", the BBC reported.
It took the activists, who were arrested afterwards, 16 hours to reach the top of the 1,016ft, 87 storey building.