London mayor Sadiq Khan has urged the government to commit to replacing EU funds which could disappear when the UK leaves the bloc.
Groups are currently applying for money from the £22m European Regional Development Fund (ERDF), which is targeted at promoting small businesses and environmental initiatives.
However, the mayor's office is concerned that this funding is under threat, and that the £22m will be the last payment made available to the capital.
Thirty-three London projects have benefited from a total of more than £50m from the ERDF since 2016, and a further seven projects are currently negotiating funding worth £12m.
Khan said: "London has benefited enormously over recent years through European funding streams such as this and I have vowed to do all I can to protect our great city from the impact of Brexit.
"One of the reasons that this fund has been so successful is because we are able to distribute the money where it is most needed."