German filings showed the fund’s short position increased to 0.72 per cent of the bank’s outstanding shares, up from 0.63 per cent earlier in the week.
The price at which the shares were sold was not disclosed. A short position means an investor bets the share price will fall by selling borrowed shares.
Shares in the bank closed down one per cent in Frankfurt.
Commerzbank announced the latest stages of its business overhaul last week, slashing almost 10,000 jobs and halting dividend payouts.
The lender is looking to focus on digitisation and automation to boost efficiency over the next four years.
Marshall Wace is also shorting Germany’s biggest lender, Deutsche Bank.
It’s expected the fine will be negotiated lower however.