The petition, which was started by Leave supporter William Oliver Healey roughly a month before the vote even took place and has received more than 4m signatures, called on government to "implement a rule that if the remain or leave vote is less than 60 per cent based a turnout less than 75 per cent there should be another referendum".
The debate will begin at 4:30pm and will be opened by Ian Blackford, Scottish National Party MP for Ross, Skye and Lochaber and member of the petitions committee.
Motions hosted on parliament's petitions websites which secure at least 10,000 signatures receive a government response, while those which gain 100,000 signatures or more will be considered for debate.
"The Prime Minister and government have been clear that this was a once in a generation vote and, as the Prime Minister has said, the decision must be respected," wrote the Foreign and Commonwealth Office in its reply, issued while David Cameron was still in Number 10. "We must now prepare for the process to exit the EU and the government is committed to ensuring the best possible outcome for the British people in the negotiations."
Other petition topics which successfully made their way to a parliamentary debate include government spending on referendum materials, blocking US Presidential hopeful Donald Trump from the UK and retrospective changes to student loan agreements.
It hasn't been smooth sailing into parliament for the referendum rules petition either, with so many people flocking to digitally sign their name on 24 June that the website crashed.