Late night revellers will be able to make their way through the capital on the underground at weekends throughout the night from 12 September.
Transport for London estimates that journeys will be cut by around 20 minutes, on average, and will boost the capital's economy by £360m, creating around 2,000 jobs.
The map highlights the specific Tube lines - the Central, Jubilee, Northern, Piccadilly and Victoria - and which parts will be running the new service on Friday and Saturday night.
While welcomed by Londoners and matching the city with places like New York and Berlin for night time services, the Night Tube has been bitterly disputed by drivers who are mounting strikes against the move.
“The introduction of the Night Tube is a historic step in our modernisation of the Underground. The Night Tube map shows our customers exactly when and where they can use this landmark service, which will make their late night journeys quicker and easier than ever before," said London Underground's strategy director Gareth Powell.
"As well making life easier for people, the Night Tube will also boost London’s night time economy - supporting thousands of jobs and stimulating hundreds of millions of pounds in economic growth.”
London mayor Boris Johnson and chancellor George Osborne have already confirmed a wider rollout of a 24 hour service across the rest of the Tube and Overground network from 2017.